Sytnhesis of organoboron amide-ester branched derivatives of poly(Itaconic anhydride-alt-2-Vinyl-1,3-dioxolane) and cancer cells interaction studies

Authors : Gülten ÖZÇAYAN

Pages : 159-171

Doi:10.30728/boron.515517

View : 20 | Download : 10

Publication Date : 2019-12-31

Article Type : Research Paper

Abstract :New organaboron amide-ester branched derivatives of poly(itaconic anhydride- alt -2-vinyl-1,3-dioxolane) are synthesized by amidolysis and grafting reactions, respectively. The structure and composition of the synthesized compounds are characterized by FTIR-ATR (Fourier Transform-Infrared-Attenuated Total Reflection) and 1 H ( 13 C) NMR (Nuclear Magnetic Resonance) spectroscopy. These novel functionalized boron containing biopolymers are interacted with HeLa (human cervix carcinoma cell) cancer cells and L929 Fibroblast ( normal) cells. Their anticancer properties are investigated using a combination of different biochemical analysis methods. It is found that both the values of the cytotoxicity and necrotic indexes are increased due to organoboron linkage but these indexes of organoboron amide-ester derivative are visibly decreased because of a -Hydroxy- ω - methoxypoly (ethylene oxide) (PEO) biocompatibilization. The results of these studies allow us to utilize PEO branched derivatives of synthesized organoboron copolymers (up to 200 µg mL -1 ) as therapeutic potential functional copolymer drugs in cancer chemotherapy and boron-neutron capture therapy (BNCT).
Keywords : biopolymers, cytotoxicity, itaconic anhydride, organoboron copolymers, vinyl dioxolane