Evaluation of 2-formylphenylboronic and 3-chlorophenylboronic acid derivatives for in vitro cytotoxicity and cell migration

Authors : Bükay Yenice Gürsu, Betül Yılmaz Öztürk, İlknur Dağ

Pages : 173-180

Doi:10.30728/boron.1493431

View : 59 | Download : 87

Publication Date : 2024-12-31

Article Type : Research Paper

Abstract :Wound treatment and skin regeneration are complex processes, and non-healing wounds pose a major socioeconomic burden in terms of health. Effective and alternative approaches are needed for successful wound management. Although boronic acid derivatives have been reported to have positive and strong effects on wound healing, phenyl-substituted boronic acid derivatives can be used as more regenerative and effective compounds in healing. In this study, the in vitro cytotoxic effects of 2-formylphenylboronic acid and 3-chlorophenylboronic acid on L929 fibroblast cell lines were investigated using WST-8 analysis, and their wound healing effects were investigated by cell migration test. Our data reveal concentration-dependent effects of both boronic acid derivatives. For 2-formylphenylboronic acid, dosage applications between 3.90-31.25 μg/ml showed a viability of 84% and above, and at higher concentrations, the viability was found to be 5-10%. For 3-chlorophenylboronic acid, a viability rate of 64-109% is observed as a result of dosage applications between 3.90-250 μg/ml, while the percentage of viability decreases to 17% at a concentration of 500 μg/ml. Cell migration test data show that the effects of phenylboronic acid (PBA) derivatives in terms of cell migration increase as time increases, and the effect of 2-formylphenylboronic acid at the 24th hour is quite effective in terms of cell migration. Since the wound healing effect of PBA derivatives is concentration dependent, it should be taken into consideration that the use of high concentrations may be toxic.
Keywords : 2-formil fenil boronik asit, 3-kloro fenil boronik asit, in vitro sitotoksisite, yara iyileşmesi