Chromatographic Determination of Essential Oil Components and Antioxidant Capacity of Mentha dumetorum Plant

Authors : Yakup Canbay, Mahfuz Elmastas

Pages : 13-13

View : 22 | Download : 42

Publication Date : 2024-12-31

Article Type : Other Papers

Abstract :This thesis study was designed to investigate the determination of the components and antioxidant capacity of the essential oil of the M.Dumetorum plant grown in Turkey. Essential oil was obtained by water distillation using the Clevenger apparatus. Essential oil analyzes of the plant were determined by the GC-MS method. Antioxidant activity was evaluated by free radical scavenging method (DPPH•), ABTS•+ radical scavenging effect determination and CUPRAC method. A total of 52 components were identified in Mentha dumetorum essential oil. Linalol (29.17%) and geraniol (14.40%) were detected as the main components in the essential oil. In all tests, antioxidant activity was found to increase with the increase of extract and standard concentration. The IC50 (effective concentration) value, which indicates the amount of antioxidant spent to reduce the initial DPPH• concentration by 50%, was detected as 10,843.49 μg/mL in the essential oil. The IC50 value of Trolox, used as a standard antioxidant, was determined as 4.29 μg/mL. In the results of the ABTS•+ radical scavenging effect determination results, the IC50 value of the essential oil was determined as 6,156.02 μg/mL. The IC50 value of the Trolox compound was determined to be 3.33 μg/mL. In the Copper(II) Ion Reduction Antioxidant Capacity Method (CUPRAC) analysis, the reducing power of Mentha dumetorum essential oil at a concentration of 50 μL/mL was determined to be 0.53 mmol TE/L. According to the results obtained, the antioxidant activity of M.dumetorum essential oil is quite low compared to the standard. According to the research results, the antioxidant effect increased similarly as the level or amount of essential oil increased, but it was observed that the antioxidant activity of M.dumetorum essential oil was much lower compared to the standard.
Keywords : Mentha dumetorum, Uçucu yağ, Antioksidan aktivite