Studies on Sterile Peg (Polyethylene Glycol)-Lated Liposomal Cisplatin-Curcumin in Lymph Cancer Cell Line

Authors : Helya Khosropanah, İsmail Aslan

Pages : 10-10

View : 17 | Download : 26

Publication Date : 2024-12-31

Article Type : Other Papers

Abstract :In this study, a formulation comprising PEGylated liposomal cisplatin-curcumin was developed for the treatment of lymphoma. It is anticipated that this formulation could offer a therapeutic approach with reduced adverse effects and enhanced efficacy for patients diagnosed with this malignancy. Aim: The objective of this study is to develop an optimal formulation of PEGylated liposomal cisplatin-curcumin that demonstrates suitable stability, pH, and osmolarity for intravenous administration, while minimizing cytotoxicity. Materials and Methods: Six samples were prepared sequentially, starting with a basic phospholipid material and progressively incorporating additional components into each subsequent sample to formulate PEGylated liposomal cisplatin-curcumin. The zeta potential and pH were measured for each formulation, and the osmolarity was assessed for one of the samples. Cell viability was assessed via the MTT assay to evaluate the cytotoxic potential of each sample. Results: The pH of the formulations ranged from 3.717 to 7.090. Additionally, the zeta potential varied between -15.1 mV and -2.24 mV. The osmolarity of one sample was measured at 427 mOsmol/kg. Cell viability across samples ranged from approximately 40% to 70%. Conclusion: In terms of pH, the optimal formulation intended for intravenous injection exhibits a pH of 4.170, implying a moderate risk profile for administration. Additionally, the most stable formulation had a zeta potential of -15.1 mV. Furthermore, the osmolarity measured for one sample was 427 mOsmol/kg, demonstrating a low risk for intravenous administration. Moreover, sample 5, composed of 10 grams from the preceding four samples, showed the lowest cytotoxicity at approximately 74% of average cell viability.
Keywords : Lipozom, Sisplatin, Kurkumin, PEGilasyon, Lenfoma