Comparison of Microwave, Ultrasonic Bath and Homogenizer Extraction Methods on the Bioactive Molecules Content of Green Tea (Camellia Sinensis) Plant.

Authors : Hafize DİLEK TEPE, Fatma DOYUK

Pages : 87-95

View : 14 | Download : 9

Publication Date : 2023-03-28

Article Type : Research Paper

Abstract :In this study, the effects of three different extraction methods on the antioxidant capacity, phenolic component, volatile organic molecule and amino acid contents of green tea insert ignore into journalissuearticles values(Camellia sinensis); leaves were compared. Microwave digestion assisted extraction insert ignore into journalissuearticles values(MDAE);, ultrasonic bath assisted extraction insert ignore into journalissuearticles values(UBAE); and homogenizer assisted extraction insert ignore into journalissuearticles values(HAE); methods were used as extraction methods. When the MDAE, UBAE and HAE extraction methods were compared according to the ferric reducing antioxidant power insert ignore into journalissuearticles values(FRAP); antioxidant activity results, 51.95, 50.9 and 13.8 TE/g dry weight insert ignore into journalissuearticles values(DW); results were obtained in green tea plant, respectively. DPPH activity in green tea was found to be 94.65, 69.7 and 36.35 TE/g dry weight in microwave extraction, ultrasonic bath and homogenizer extractions, respectively. Analysis in green tea leaves as a result of gas chromatography-mass spectrometry insert ignore into journalissuearticles values(GC-MS);, caffeine, phytol, palmitic acid, beta.-monoglyceride, 13-docosenamide, insert ignore into journalissuearticles values(z);- and phytol were identified in all three extractions. Fourteen amino acid types were determined in green tea leaves by liquid chromatography-diode array detection insert ignore into journalissuearticles values(HPLC-DAD);. Especially aspargine, glutamine, alanine, serine and L-theanine were determined in very high amounts. When the analysis data were compared, it was determined that the best results were in MDAE>UBAE>HAE, respectively
Keywords : Extraction, green tea, HPLC DAD, amino acid, phenolic compounds, LC MS MS