Effect of Resveratrol and Quercetin on Intestinal Ischemia Reperfusion Injury in Rats

Authors : Işıl Yağmur, Selen Dindar, Rabia Tural, Aslı Yaylali, Atila Yoldaş, Fatma Inanç Tolun, Hasan Dağli

Pages : 467-472

Doi:10.36516/jocass.1303016

View : 125 | Download : 91

Publication Date : 2023-12-31

Article Type : Research Paper

Abstract :Aim: Intestinal ischemia reperfusion (I/R) injury is an emergency condition with a high mortality rate and early diagnosis is very difficult. In this study, we aimed to examine the biochemical and histopathological effects of resveratrol and quercetin on intestinal I/R injury model. Material and Method: In our study, 56 male Sprague-Dawley rats weighing 250-300 g were randomly divided into 7 groups consisting of 8 rats. Groups were control group (group 1), saline group (group 2), ethanol group (group 3), resveratrol group (group 4) (30mg/kg), quercetin group (group 5) (30mg/kg), resveratrol+quercetin group A (group 6) (15 mg/kg+15 mg/kg), resveratrol+quercetin group B (group 7) (30 mg/kg+30 mg/kg). At the end of the experiment rats intestinal tissues were divided into 2 parts for biochemical and histopathological examination. Total oxidant level (TOS), total antioxidant level (TAS), total thiol [(-SH)+(-S-S-)] (TT), native thiol [-SH] (NT), and protein content levels were measured spectrophotometrically, oxidative stress index (OSI) and disulfide [-S-S-] levels were calculated. Results: A statistically significant difference was found between the groups in terms of TOS, OSI, TT, NT and disulfide levels (p <0.05). No statistically significant difference was observed between the groups in terms of TAS levels (p˃0.05). A significant improvement in histopathological scoring was observed in all treatment groups compared to saline and ethanol groups (p<0.05). Conclusion: Resveratrol and quercetin have protective effects in reducing oxidative stress in intestinal I/R damage.
Keywords : İschemia reperfusion, resveratrol, quercetin, intestinal tissue damage

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