The relationship between carbonic anhydrase-III expression and oxidative stress in brown adipose tissue

Authors : Cemil KAHRAMAN, Ahmet ALVER, Ayşe ŞENTÜRK, İmran İnce AKÇA

Pages : 361-368

Doi:10.5798/dicletip.497874

View : 22 | Download : 12

Publication Date : 2018-12-13

Article Type : Research Paper

Abstract :Objective: High-fat foods increase adipose tissue size, and induce obesity. Although carbonic anhydrase III is abundantly found in brown adipose tissue, its function is not fully defined. In this study, we investigated the relationship between carbonic anhydrase III enzyme mRNA expression and malondialdehyde, oxidative stress marker, in brown adipose tissue of rats that were fed high-fat diets. In addition, we investigated potential effect of N-acetylcysteine as an antioxidant in this relationship. Methods: In our study three experimental groups were formed and each contained 6 rats (control, obese, and antioxidant groups). The experimental groups were fed for a duration of 85 days with high fat diets. In these groups, carbonic anhydrase III mRNA expression, total carbonic anhydrase hydratase activitie, and malondialdehyde levels were measured in brown adipose tissues dissected from rat scapula regions. Results: According to our findings, carbonic anhydrase III mRNA expression was higher in the obese group than in the control group (p = 0.004), and malondialdehyde levels were lower in the obese group than in the control group (p = 0.03). It was observed that carbonic anhydrase III mRNA expression was higher in the antioxidant group than in the control group (p = 0.006), and malondialdehyde levels were lower in the antioxidant group than in the control group (p = 0.006). In addition, in the obese group carbonic anhydrase III mRNA expression was higher than in the antioxidant group (p=0.01). Conclusion: In brown adipose tissue of rats that were fed high-fat diets, this study showed that the carbonic anhydrase III mRNA expression increased and the malondialdehyde level decreased.
Keywords : Carbonic anhydrase III, brown adipose tissue, obesity, N acetylcysteine