Comparison of Two Different Serological Methods in The Diagnosis of Rotavirus and Adenovirus in Stool Samples

Authors : Oktay İlhan, Mehmet Tevfik Bayraktar, Bashar Ibrahim

Pages : 262-269

Doi:10.46237/amusbfd.1275682

View : 100 | Download : 95

Publication Date : 2024-09-30

Article Type : Research Paper

Abstract :Objective: Rotaviruses are the most common cause of acute infectious diarrhea in children under 5 years of age worldwide. This study aims to compare the immunochromatographic and ELISA methods, determine the sensitivity and specificity of the methods used, and shed light on humanity to diagnose the disease correctly. Method: Stool samples of 1000 patients were sent to Diyarbakır Pediatric Hospital for rapid antigen testing of Rotavirus. Single-step Rotavirus and Adenovirus Co-immunochromatographic and ELISA methods were used in the examination of the samples. Samples with positive rapid test results were collected in 1.5 ml microcentrifuge tubes, and positive antigen samples collected for the ELISA test were stored at -80°C until the time of study. Results: Of 1000 stool samples analyzed, 40 (11.59%) of 345 girls and 60 (9.16%) of 655 boys had Rotavirus antigen in a total of 100 (10%) and 20 (2%) had Adenovirus antigen (P> 0.05). Viral antigen-positive cases were most frequently seen in winter months (December, January, February). There was no statistically significant difference between the data in terms of age and gender (P <0.05). Conclusion: In this study, Rotavirus was detected in one-tenth of diarrhea cases in pediatric patients and it was revealed that it increased seasonally in winter months.
Keywords : Rotavirüs, Gastroenterit, ELİSA, Immünokromatografik, Pediatri

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