INVESTIGATION OF THE ACTION MECHANISM OF AMMONIUM PYRROLIDINE DITHIOCARBAMATE ON RAT TRACHEA SMOOTH MUSCLE CONTRACTION-RELAXATION RESPONSE

Authors : Hayri DAYIOĞLU, Ayhan YILMAZ, Merve AKTAŞ, Fatih ALAN, Sinan DARCAN

Pages : 12-27

View : 23 | Download : 14

Publication Date : 2020-12-31

Article Type : Research Paper

Abstract :Ammonium pyrrolidine dithiocarbamate (APDTC) is a suppressive thiol compound of Nuclear Factor kappa B (NF-κB) and works to prevent infections, regulate oxidation, prevent cell death, affects on viruses. Therefore, it is recommended to avoid harmful practices and to give importance to active substance research in medical ethics. In postoperative care, respiratory system relaxation treatments are important. There is insufficient research into how the functions of APDTC are regulated in human and animals. In our study, we aimed to evaluate the function of APDTC in rat trachea smooth muscle. 70 male Wistar albino rats were used. The rats were euthanized by giving anesthesia and after applying cervical dislocation the trachea was separated, connective tissues of trachea were separated and placed in Krebs solution. Potassium chloride (KCl), Acetylcholine (ACh), APDTC, Atropine, Phentolamine, Propranolol, Nifedipine and Tetraethylammonium (TEA) were added in to the solution. Eventually, APDTC produced a relaxation response in the tracheal smooth muscle induced by ACh, but this relaxation was not statistically significant. In our study, Half maximal effective concentration (EC50) dose was found to be ineffective. APDTC did not induce L-type Ca2+ channel and K+ channel receptors to differentiate relaxation response by producing a cholinergic-adrenergic effect. We think that APDTC did not affect these channels. Further studies with the use of different doses are recommended.
Keywords : APDTC, trachea, adrenergic, cholinergic, Ca2 channels, K channels