Effectiveness of In Vitro and In Vivo Tests for Screening of Tomato Genotypes against Drought Stress

Authors : Gökçe AYDÖNER ÇOBAN, Hakan ALTUNLU, Ayşe GÜL

Pages : 143-150

Doi:10.20289/zfdergi.835798

View : 53 | Download : 19

Publication Date : 2020-12-31

Article Type : Research Paper

Abstract :Objective: The aim of this study was to investigate possibilities of fast screening of local tomato genetic material against drought. Material and Methods: In this study including in vitro and in vivo tests, seeds of 92 tomato genotypes were used and drought stress was induced by polyethylene glycol 6000 (PEG) at 4%. Firstly, seed germination test was made; and 5 genotypes with higher and 5 genotypes with lower performances were selected according to the evaluation made at 14 days. These genotypes were tested by water culture. Full drought dose was Ψs= -1.0 MPa and 48 hours after the full dose application, morphological and physiological properties of the plants were determined. The genotypes tested were classified by weighted ranking method, based on the changes in the PEG treatment compared to the control. Results: The correlation coefficient (r: 0.824) for the relationship between the variation (%) of in vitro vigour index and the total score of weighted ranking in water culture was significant. As a result of this study; 97:TR70707, 68:TR69163 and 60: TR68515 in the genetic pool tested, were determined as the most tolerant genotypes against drought. Conclusion: It was concluded that in vitro seed germination test can be used for pre-screening of large numbers of genotypes in response to drought stress.
Keywords : Solanum Lycopersicum, kuraklık, polietilen glikol 6000

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