Antimicrobial Peptide Conjugated PEGDA Based Hydrogel for Wound Dressing Applications

Authors : Buse Sezer, Eda Bilgiç, Günnur Pulat

Pages : 1511-1520

Doi:10.35414/akufemubid.1673131

View : 94 | Download : 252

Publication Date : 2025-12-03

Article Type : Research Paper

Abstract :Currently, management of chronic wounds pose a significant challenge. In the treatment of chronic wounds, hydrogel based wound dressings considered as an effective approach. Poly(ethylene glycol) diacrylate (PEGDA) hydrogel is an advantageous hydrogel due to its properties such as tunable mechanical properties and biocompatibility. However, PEGDA hydrogels lack antimicrobial properties. Therefore, the development of new antibacterial strategies combined with PEGDA hydrogel can be effective in overcoming the problems associated with chronic infected wounds. Herein, we developed an antimicrobial wound dressing by conjugating antimicrobial peptide KSL-W (KKVVFWVKFK) to PEGDA hydrogel via EDC/NHS chemistry. The antimicrobial activity was evaluated against MRSA and multi-drug resistant (MDR) P. aeruginosa in their planktonic form. Furthermore, cytotoxicity and wound healing were assessed using L929 mouse fibroblast cells. Developed KSL-W peptide conjugated PEGDA hydrogels showed strong antimicrobial activity against MRSA and MDR P. aeruginosa with a bacterial reduction of 2.44 and 1.15 log, respectively. Furthermore, developed hydrogels showed no undesired toxic effect against healthy mammalian cells and supported wound healing. Our findings provide insight into the development of antimicrobial hydrogels for biomedical applications to develop more effective antimicrobial wound dressing solutions.
Keywords : Biyomalzemeler, yara örtüleri, hidrojeller, poli(etilen glikol) diakrilat, antimikrobiyal peptid

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