Engineered Neuronal Spheroids Using Sacrificial Materials for 3D Advanced Bioprinting

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Erciyes Üniversitesi Fen Bilimleri Enstitüsü Dergisi
Cilt: 41 Sayı: 2
Engineered Neuronal Spheroids Using Sacrificial Materials for 3D Advanced Bioprinting

Engineered Neuronal Spheroids Using Sacrificial Materials for 3D Advanced Bioprinting

Authors : Dr. Tuğrul Tolga Demirtaş

Pages : 447-458

View : 49 | Download : 24

Publication Date : 2025-08-30

Article Type : Research Paper

Abstract :The development of physiologically relevant 3D tissue models remains a challenge in neural tissue engineering. This study explores the use of sacrificial materials, such as sodium alginate, in the 3D bioprinting of SH-SY5Y spheroid constructs to enhance structural and functional properties. Spheroids with an average diameter of 80–100 µm were formed and assessed for viability, proliferation, and differentiation using Live/Dead, Presto Blue, and immunocytochemical staining techniques. Results indicated successful spheroid formation with over 90% viability, optimized bioprinting with consistent filament patterns, and a proliferation plateau by day 14. Confocal microscopy on day 14 revealed intense MAP2 and TAU expression, signifying robust dendritic and axonal development, alongside extended neurite networks. These findings underscore the potential of sacrificial material-based bioprinting to advance 3D neural tissue models for research and therapeutic applications
Keywords : 3B biyobaskı, Nöronal sferoid, Destek (kurban) malzeme

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