Lectin-Based Histochemical Analysis of Sugar Residues in the Gastrointestinal Tract of New Zealand White Rabbit

Authors : Seval Türk, Mustafa Öztop

Pages : 65-72

Doi:10.32707/ercivet.1619736

View : 81 | Download : 70

Publication Date : 2025-08-08

Article Type : Research Paper

Abstract :The gastrointestinal system of mammals exhibits interspecific variability both physically and functionally. The diversity in the gastrointestinal system reflects unique and functional traits among vertebrate animals. Lectin histochemistry is a method that uses lectins to identify and attach to glycosidic linkages in polysaccharides, glycoproteins, and glycolipids. This particular binding enables the identification of complex structures by determining terminal sugars, hence elucidating physiological or pathological alterations in cells, intercellular relationships, and intracellular transport mechanisms. The present study aimed to identify and illustrate the distribution and density of glycans throughout the gastrointestinal system of the New Zealand White rabbit, spanning from the stomach to the rectum, utilizing lectin histochemistry. Paraffin sections of Bouin-fixed rat tissues were taken 5-μm thick and were examined for the binding of lectins specific to GalNAc (HPA), Gal (PNA), GlcNAc (WGA), and mannose and/or glucose (Con A) using the lectin histochemical method. The lectin binding patterns in the stomach both the small and large intestines exhibited alterations, signifying a diverse composition of carbohydrates. The data indicate that mucin glycosylation differs across various anatomical locations and likely represents a responsive mechanism tailored to local physiological requirements.
Keywords : Tavşan, Bağırsak, gastrointestinal sistem, lektin histokimyası, mide

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