Investigation of virulence factors of shiga toxin-producing Escherichia coli strains isolated from sheep

Authors : Nuray ŞAHİN, Murat YILDIRIM, Sibel KIZIL

Pages : 36-45

Doi:10.35864/evmd.1264065

View : 35 | Download : 50

Publication Date : 2023-06-20

Article Type : Research Paper

Abstract :All over the world, Escherichia coli insert ignore into journalissuearticles values(STEC);, which produces Shiga toxin, is recognized as an important zoonotic pathogen. Ruminants, particularly cattle, are their main reservoirs, but the role of small ruminants in the epidemiology of human infections has not been extensively evaluated in many countries. In this study, it was aimed to determine the Shiga toxin subtypes of Stx1, Stx2, eae and ehxa genes of Shiga toxin producing isolates within Escherichia coli strains in sheep and to determine the comparative effect of variables such as race and sex on them. In this study, virulence genes of STEC strains and Shiga toxin subtypes were identified in 215 E. coli isolates isolated from 272 rectal swab from 12 different sheep herds in and around Ankara. Of the 215 isolates, only 19 insert ignore into journalissuearticles values(8.84%); Stx1, 3 insert ignore into journalissuearticles values(1.4%); Stx2, 5 insert ignore into journalissuearticles values(2.33%); eae and 9 insert ignore into journalissuearticles values(4.19%); ehxa genes, 13 insert ignore into journalissuearticles values(6%, 05); Stx1-Stx2-ehxa, 5 insert ignore into journalissuearticles values(2.33%); Stx1-eae -ehxa, 3 insert ignore into journalissuearticles values(1.4%); Stx2-eae-ehxa together and 1 insert ignore into journalissuearticles values(0.47%); Stx1- eae, 30 insert ignore into journalissuearticles values(13.95%); Stx1- ehxa, 1 insert ignore into journalissuearticles values(0.47%); Stx2- eae, 15 insert ignore into journalissuearticles values(6.98%); Stx2-ehxa and 10 insert ignore into journalissuearticles values(4.65%); eae – ehxa were detected together. Of the 101 insert ignore into journalissuearticles values(46.98%); isolates, none of the Stx1, Stx2, eae and ehxa genes were detected. In stx1 subtyping in sheep, a high rate insert ignore into journalissuearticles values(82.35%); of stx1c was isolated. In stx2 subtyping stx2f insert ignore into journalissuearticles values(%62,86);, stx2c insert ignore into journalissuearticles values(%54,29);, stx2b insert ignore into journalissuearticles values(%45,71);, stx2d insert ignore into journalissuearticles values(%22,86);, stx2h insert ignore into journalissuearticles values(%22,86); subtypes are more common in different combinations. Detected at a high rate. These data indicate the presence of STEC in sheep and their potential to cause serious infection in humans
Keywords : Shiga Toksin Üreten E coli STEC, Stx1, Stx2, Alt Tip, Koyunlar