Eucalyptol (1.8-cineole) attenuates gentamicin-induced liver injury

Authors : Özhan Karataş, Filiz Kazak, Gökhan Akçakavak, Halil Alakuş, Ahmed Abduljabbar, Ömer Kırgız, İbrahim Alakuş, Bahadır Kılınç, Zeynep Çelik, Mehmet Tuzcu

Pages : 133-141

Doi:10.35864/evmd.1549327

View : 59 | Download : 77

Publication Date : 2025-01-03

Article Type : Research Paper

Abstract :Gentamicin (GM), which is in the aminoglycoside antibiotic class, is frequently preferred today in the treatment of diseases caused by gram-negative bacteria. However, its significant side effects on liver and kidney functions limit its clinical usefulness. The antioxidant and anti-inflammatory medical activities of eucalyptol (EUC, 1.8-cineole) have been reported in different studies. This study aimed to evaluate the effects of EUC on GM-induced hepatotoxicity. The study groups are consisted of control (C), EUC, GM and GM + EUC, and there were 7 rats in each group. At the end of the study, the rats were euthanized under appropriate conditions and samples were collected and biochemical, histopathological and immunohistochemical analyzes were performed. It was determined that there was a important increase in serum alanine aminotransferase (ALT), aspartate transferase (AST) and gamma-glutamyl transferase (GGT) enzymes in the GM group relative to the C group (p<0.001). GM application is determined that it caused histopathological damage in livers. Additionally, immunohistochemically, it caused an important increase in the expressions of 4-hydroxynonenal (4-HNE), 8-hydroxy-2\\\'-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) in rats in the GM group relative to the C group (p<0.001). Eucalyptol application (GM+EUC) regulated the increase in serum ALT, AST and GGT enzymes. It also attenuated 4-HNE, 8-OHdG and MDA expressions. It attenuated the histopathological damage caused by GM application. The results of this study revealed that EUC showed antioxidative, protective and curative efficacy in GM-induced liver damage.
Keywords : gentamisin, hepatotoksisite, MDA, okaliptol (1.8-sineol), 8-OHdG