Serum Inflammatory Cytokines and Growth Factors in Patients with Sjögren’s Syndrome and Diabetes Mellitus

Authors : Şule Nur ACAR DUYAN, Banu BOZKURT, Ali UNLU, Sema YİLMAZ, Yalçın KARAKÜÇÜK

Pages : 360-366

Doi:10.54005/geneltip.1314282

View : 94 | Download : 84

Publication Date : 2023-08-31

Article Type : Research Paper

Abstract :Abstract Background/ Aims: Sjögren’s syndrome insert ignore into journalissuearticles values(SS); and diabetes mellitus insert ignore into journalissuearticles values(DM); are common causes of dry eye disease insert ignore into journalissuearticles values(DED); and autologous serum is used when artificial tears are not sufficient. Our aim was to compare serum growth factor and inflammatory cytokine levels of SS and DM patients with the healthy individuals. Methods: Twenty-three SS patients insert ignore into journalissuearticles values(mean age 52.70±7.55 years);, 25 DM patients insert ignore into journalissuearticles values(mean age 56.68±6.53 years);, and 23 healthy subjects insert ignore into journalissuearticles values(mean age 51.70±9.14 years); were included in the study. After detailed ophthalmological examination, Schirmer test, tear break-up time insert ignore into journalissuearticles values(TBUT); and Ocular Surface Disease Index insert ignore into journalissuearticles values(OSDI); scores were measured. Serum levels of six different proinflammatory interleukins insert ignore into journalissuearticles values(IL);, five growth factors, matrix metalloproteinase-9, and fibronectin were measured by immunoassay. One-way ANOVA or Kruskal-Wallis tests and Dunn-Bonferroni post hoc analysis were used for comparison and p<0.05 was considered significant. Results: Schirmer test and TBUT were significantly lower in the SS group insert ignore into journalissuearticles values(2.08±1.72 mm/5 min and 3.08±2.08 s); than in the DR insert ignore into journalissuearticles values(10.24±4.63 mm/5 min and 4.20±3.09 s); and control groups insert ignore into journalissuearticles values(13.30±5.95 mm/5 min and 9.00±1.75 s); insert ignore into journalissuearticles values(p<0.001);. Among the parameters studied, mean serum IL-23 level was significantly higher in the SS group insert ignore into journalissuearticles values(156.66±207.94 pg/mL); than in the DM and control groups insert ignore into journalissuearticles values(73.48±95.91 and 69.59±105.39 pg/mL, respectively); insert ignore into journalissuearticles values(p<0.05);. Serum insulin-like growth factor 1 insert ignore into journalissuearticles values(IGF-1); level was lowest in DM patients insert ignore into journalissuearticles values(DM: 12.89±21.09, SS: 30.77±19.85, and control: 27.08±21.93 ng/mL); insert ignore into journalissuearticles values(p<0.05);. Sjögren’s syndrome disease activity index insert ignore into journalissuearticles values(ESSDAI); showed a negative correlation with TBUT and a positive correlation with IL-1, IL-2 and fibronectin insert ignore into journalissuearticles values(p<0.005);. Conclusions: Except IL-23 and IGF-1, the contents of serum obtained from patients with SS and DM are similar with the healthy individuals. Therefore, autologous serum seems to be a good option to replace deficient tear fluid in these subjects.
Keywords : İnflamatuar sitokinler, Büyüme Faktörleri, Sjögren sendromu, Diabetes mellitus, Otolog serum