Optimization of Microwave-assisted Extraction of Phenolics from Organic Strawberry Using Response Surface Methodology

Authors : Aysel ELİK, Derya KOÇAK YANIK, Fahrettin GÖĞÜŞ

Pages : 143-154

Doi:10.29050/harranziraat.321124

View : 34 | Download : 13

Publication Date : 2017-06-19

Article Type : Research Paper

Abstract :The effects of microwave- assisted extraction (MAE) were investigated on extraction of phenolic compounds from strawberry fruit. Response surface methodology (RSM) was used to optimize the extraction conditions. A face-centered central composite design (FCCCD) was employed to determine the effects of independent variables such as microwave power (100-300 W), extraction time (2-16 min) and solvent to sample ratio (5:1-25:1 mL g -1 ) on the extraction yield and total phenolic content (TPC). Optimized conditions were determined as 265 W of microwave power, 2 min of extraction time and 24:1 mL g -1 of solvent to sample ratio. The maximum predicted extraction yield and TPC under the optimum conditions were 8.23 % and 19.65 mg GAE g -1 dry strawberry, respectively . Total anthocyanin content (TAC), DPPH·EC 50 and FRAP values of extracts produced at optimum conditions were determined as 2.3 mg Cyn-3-glu g -1 dry strawberry , 1.67 mg dry strawberry mL -1 and 197.83 μmoles TE g -1 dry strawberry, respectively.
Keywords : Microwave assisted extraction, Strawberry, Phenolics, Response surface methodology