Isolation of bacterial isolates from soil samples and determination of their in vitro antagonistic potential against chickpea wilt disease agent Fusarium oxysporum f. sp. ciceris

Authors : Murat OZTURK, Ali ENDES

Pages : 64-72

Doi:10.29050/harranziraat.1216304

View : 22 | Download : 16

Publication Date : 2023-03-24

Article Type : Research Paper

Abstract :In 2021, post-harvest soil samples were taken from fields where chickpea and wheat were grown in Yozgat province to isolate bacteria. Of 74 isolates obtained from soil samples, 14 nonpathogenic isolates were evaluated for their inhibitory potential against two pathogenic isolates insert ignore into journalissuearticles values(YBUFoc9 and YBUFoc2); of Fusarium wilt disease agent Fusarium oxysporum f. sp. ciceris. Antagonistic bacterial isolates were identified as Bacillus spp. by Blastn analysis based on their 16S rDNA nucleotide sequences. Among them, 2 isolates were identified as B. amyloliquefaciens insert ignore into journalissuearticles values(BM23 and BM40);, 3 isolates as B. subtilis insert ignore into journalissuearticles values(BM8, BM32, and BM105);, 6 isolates as B. cereus insert ignore into journalissuearticles values(BM10, BM69, BM70, BM104, BM111 and BM215);, 2 isolates as B. megaterium insert ignore into journalissuearticles values(BM44 and BM135); and 1 isolate as B. pumilus insert ignore into journalissuearticles values(BM28);. No effective isolate of any genus other than Bacillus spp. was found. They inhibited mycelial growth of isolates YBUFoc9 and YBUFoc2 from 0 to 78.50% and from 0 to 58.33%, respectively. Among the 5 different species, B. amyloliquefaciens insert ignore into journalissuearticles values(BM23 and BM40); and B. subtilis insert ignore into journalissuearticles values(BM8, BM32, and BM105); showed the strongest inhibitory activity against the two fungal isolates under in vitro conditions. In further studies, the biocontrol properties of the most effective isolates insert ignore into journalissuearticles values(BM23, BM40, BM8, BM32 and BM105); will be investigated and compared with approved fungicides for their fungicidal activity.
Keywords : Fusarium solgunluğu, Nohut, Bacillus spp, Biyolojik mücadele