Evaluation of Serum Amino Acid and Carnitine Profile in Dogs with Transmissible Venereal Tumor

Authors : Tuğra Akkuş, Ömer Yaprakcı, Nida Özaslan

Pages : 100-105

Doi:10.31196/huvfd.1496923

View : 5 | Download : 6

Publication Date : 2024-12-18

Article Type : Research Paper

Abstract :The presented study aimed to reveal the changes in serum amino acid and carnitine profiles in dogs with transmissible venereal tumor (TVT). The study material comprised 40 female dogs ranging in age from 3 to 5 years. The dogs were divided into two groups based on genital organ examinations. Group 1 (n=20) consisted of healthy dogs, while Group 2 (n=20) consisted of TVT-positive dogs. Blood samples were taken from dogs in both study groups, and serum was obtained. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used for the determination of carnitine and amino acid profiles. The obtained data were compared using an independent samples t-test. The serum amino acid profiles of Lysine, Aspartic Acid, Tyrosine, Asparagine, Alanine, Arginine, Citrulline, Glutamic Acid, Glycine, Methylglutaryl, Phenylalanine, and Ornithine were found to be lower in the TVT group (P<0.05). The serum carnitine profiles of C0 (Free carnitine), C2 (Acetylcarnitine), C4 (Butyrylcarnitine), C4-DC (Methylmalonylcarnitine), C5 (Isovalerylcarnitine), C5:1 (Tiglylcarnitine), C5-OH (3-Hydroxyisovalerylcarnitine), C5-DC (Glutarylcarnitine), C6 (Hexanoylcarnitine), C6-DC (Adipoylcarnitine), C8 (Octanoylcarnitine), C8:1 (Octenoylcarnitine), C8-DC (Suberoylcarnitine), C10 (Decanoylcarnitine), C10:1 (Decenoylcarnitine), C12 (Dodecanoylcarnitine), C14 (Myristoylcarnitine), C14:1 (Myristoylcarnitine), C14:2 (Tetradecadienoylcarnitine), C18:1 (Oleoylcarnitine), C18:2 (Linoleylcarnitine), and C18:1-OH (Hydroxyoleylcarnitine) were found to be lower in the TVT group (P<0.01). The profiles of C3 (Propionylcarnitine) and C16 (Palmitoylcarnitine) were found to be higher in the TVT group (P<0.01). As a result, it was concluded that the significant changes in amino acid and carnitine values could be used as biomarkers for diagnosing TVT in dogs.
Keywords : Amino asit, karnitin, köpek, transmissible venereal tümör