Comparative Assessment of Potential Toxicity and Biochemical Properties of Rhododendron luteum and Rhododendron ponticum Flower Extracts on Artemia salina

Authors : Yeşim Dağlıoğlu

Pages : 39-50

View : 33 | Download : 49

Publication Date : 2024-12-26

Article Type : Review Paper

Abstract :The focus of this study was to comparatively investigate the toxicity of different Rhododendron species (Rhododendron luteum and Rhododendron ponticum) at different concentrations on instar IV larvae of Artemia salina (Brine shrimp). Because Rhododendron species belong to a Ericaceae family rich in bioactive compounds, which makes them highly poisonous. We investigated the effect of two different Rhododendron species on the mortality rate of instar IV larvae, the potential to induce oxidative stress, and the defense capacity of the larvae. Larvae were exposed to different concentrations of Rhododendron extracts in artificial seawater for 48–96 h in cell culture plates. Mortality rate and morphological changes were evaluated at each concentration under a stereomicroscope. In our study, Rhododendron species were found to be highly toxic to A. salina instar IV larvae and R. ponticum was more toxic than R. luteum. Particularly, the level of MDA, which indicates cell damage, increased significantly in both Rhododendron species at a concentration of 200 µg/mL and above (P<0.05). On the other hand, the level of SOD, which is a defense strategy to reduce the effect of oxidative stress, decreased with increasing concentration in Rhododendron species, but was significant only in the concentration range of 800-3200 µg/mL (P<0.05).
Keywords : MDA, oksidatif stres, Rhododendron sp., SOD, toksisite.