Cetylpyridinium Chloride Induces Resistance Genes in Candida Albicans

Authors : Banu UYGUNCAN
Pages : 68-81
Doi:10.38079/igusabder.1021179
View : 38 | Download : 14
Publication Date : 2023-04-29
Article Type : Research Paper
Abstract :Aim: The antimicrobial cetylpyridinium chloride insert ignore into journalissuearticles values(CPC); is used in the management of denture stomatitis-associated oral candidiasis as an alternative therapy as well as for oral hygiene. Cetylpyridinium chloride survives in the oral cavity for long periods at low doses, which fluctuates due to the dynamics of the oral cavity. In this study, it was aimed to evaluate the impact of different sub-therapeutic CPC concentrations insert ignore into journalissuearticles values(1/100, 1/200); for different time periods insert ignore into journalissuearticles values(0.,5., 2., 24., 48. h); on the expression of drug-resistance genes insert ignore into journalissuearticles values(CDR1, CDR2, MDR1, ERG11); in Candida albicans SC5314. Method: Total RNA was extracted immediately after antimicrobial exposure using the Biospeedy® Tri-Easy Isolation Kit followed by Real-Time Quantitative Reverse Transcription polymerase chain reaction insert ignore into journalissuearticles values(qRT-PCR);. The data were analyzed by the comparative 2-ΔΔCT method to calculate the relative expression of the target genes after treatment with different CPC concentrations, standardized to the housekeeping gene Actin. Results: In this study, it was found that the drug resistance gene expression levels increased after exposure to high CPC concentrations insert ignore into journalissuearticles values(1/100); for 48 h, whereas the gene expression levels were downregulated at 1/200. Conclusion: These results may provide an insight into the mechanisms of action of drug-resistance genes in Candida albicans and aid the development of future strategies for using CPC as an alternative therapy.
Keywords : Setilpridinyum klorür, ilaç direnç genleri, oral kandidiyazis, candida albicans, antimikrobiyal ajanlar

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