Electrochemical Characterization of Cholesterol Biosensor Formed by Polymer Film Based on o-Phenylenediamine and Benzoquinone

Authors : Kezban KARTLAŞMIŞ, Umut KÖKBAŞ, Levent KAYRIN

Pages : 347-360

Doi:10.38079/igusabder.1026210

View : 97 | Download : 47

Publication Date : 2023-09-09

Article Type : Research Paper

Abstract :Aim: The detection of cholesterol concentration in the blood is an important parameter in the diagnosis and follow-up of many diseases, including cardiovascular and hepatobiliary diseases. Nowadays, various methods have been used for the routine analysis of cholesterol, including spectrophotometric, high-performance liquid chromatography insert ignore into journalissuearticles values(HPLC);, and amperometric methods. Since biosensors have advantages such as high sensitivity, fast response, low cost, small size, continuous online detection, and reproducible results, it is aimed to develop a biosensor method for cholesterol determination. Methods: Polymer film immobilization was performed on the gold electrode surface electrochemically in an acetonitrile-water medium containing o-phenylenediamine and benzoquinone. Cholesterol oxidase insert ignore into journalissuearticles values(ChOx, E.C. 1.1.3.6); enzyme was immobilized on the polymer film-immobilized electrode surface by the self-forming monolayer method. Optimization and characterization studies were carried out for the determination of cholesterol with the prepared electrodes. Results: The determination of cholesterol was performed via monitoring the oxidation current of enzymatically produced H2O2 at 0.70 V Ag/AgCl. Optimum biosensor conditions were determined for phosphate buffer at 0.1M, pH 7.0, and 30°C for the Au/oPD-BQ/ChOx enzyme electrode. The linear working range is 9.8×10-6-1.1×10-2 mM, and the cholesterol detection limit is 9.8×10-6 mM. The analysis of total cholesterol in solutions was performed using the proposed Au/oPD-BQ/ChOx electrode. Conclusion: A convenient, simple, reproducible, and stable method was developed for the determination of cholesterol by immobilizing cholesterol oxidase on the prepared electrode surface through a polymer film. The sensor we designed can be expanded to improve the detection of many analytes in the clinic.
Keywords : Benzokinon, biyosensör, kolesterol, o fenilendiamin, polimer film

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