Effects of 3,3’-Diindolylmethane on Rat Kidney Tissue

Authors : Seçil Nazife Parlak, Seda Yakut, Habibe Gündoğdu, Tuba Demirci

Pages : 224-230

View : 61 | Download : 95

Publication Date : 2024-12-31

Article Type : Research Paper

Abstract :Aim: 3,3’-diindolylmethane (DIM) is an important digestive product of indole-3 carbinol (I3C) obtained from the Brassica family (broccoli, cauliflower, cabbage, etc.) of vegetables. 3,3’-diindolylmethane is a substrate with potent immune modulatory activity and antitumor, antiviral, and anti-angiogenic effects. This study aimed to evaluate the effects of DIM on rat kidney tissue using histopathologic methods. Material and Method: In the study, 36 males, 16-week-old, and 220–260 gr Wistar albino adult rats were used. Rats were divided into four equal groups: The control group received only corn oil by oral gavage. The other experimental groups received three different doses of DIM dissolved in corn oil, 10 mg/kg DIM (DIM 10 group), 50 mg/kg DIM (DIM 50 group), and 100 mg/kg DIM (DIM- 100 group, were administered via oral gavage. Oral gavages were applied to experimental groups for 53 days. At the end of the experiment, all rats were euthanized, and the kidney tissues were dissected. For histopathological examination, the kidney tissue samples were stained with hematoxylin-eosin and Masson–trichrome. Results: Our investigation revealed that the use of DIM at different doses for 53 days caused dose-dependent histopathological changes, including apoptotic to necrotic changes, interstitial inflammation to fibrotic connective tissue changes, and cast formations starting from the Henle loops and spreading to the renal tubules. Conclusion: These histopathological changes could have occurred due to a DIM-mediated increase in reactive oxygen species (ROS). Further biochemical, molecular, and ultrastructural studies are needed to clarify these findings.
Keywords : 3, 3’-diindolylmethane, indole-3 carbinol, kidney, reactive oxygen species (ROS)