The Use of HPLC-DAD Method in Determining the Quality of Honeys: 5-Hydroxymethylfurfural (HMF) Analysis

Authors : Merve Özbay, Fatma Nur Arslan, Gazi Görür

Pages : 41-45

Doi:10.55213/kmujens.1529151

View : 10 | Download : 18

Publication Date : 2024-12-15

Article Type : Research Paper

Abstract :The recognition of 5-hydroxymethylfurfural (HMF) content in honeys has been the subject of many scientific studies as it is a significant indicator of the toxicity, its processing method, storage situations and age. In this research, the amount of HMF in 95 different flower and honeydew honey samples obtained from markets, factory sales offices and local beekeepers in Turkiye was determined. High performance liquid chromatography (HPLC) method was employed to recognize HMF contents. Before sample analysis, the standard HMF solutions were prepared at different concentrations and method validation studies were carried out. The HMF content limit value of flower and honeydew honey in the Honey Communiqué (TFC/Communiqué No:2020/7) is stated as 40 mg.kg-1. According to the obtained results, the HMF contents in honey samples were found as 0.00-16.65 mg.kg-1. It was determined that the amounts of HMF were at the highest level in monofloral milk vetch honey (MH_MV) samples coded MH_MV3 and MH_MV4. Therefore, it was concluded that all of the honey samples were compatible with the Honey Communiqué in terms of HMF and had appropriate honey quality values.
Keywords : Çiçek balı, Salgı balı, 5-hidroksimetilfurfural, HPLC