INVESTIGATION OF ANTIANGIOGENIC AND APOPTOTIC EFFECTS OF GALANTAMINE OBTAINED FROM LEUCOJUM AESTIVUM ON MCF-7 BREAST CANCER CELL LINE

Authors : Ali Tarık İNCE, Doç Dr Funda KARABAĞ, İbrahim BULDUK

Pages : 457-465

Doi:10.18229/kocatepetip.1178657

View : 32 | Download : 45

Publication Date : 2023-10-09

Article Type : Research Paper

Abstract :OBJECTIVE: The current study aims to investigate the antiangiogenic and apoptotic effects of galantamine in breast cancer cells and to add new information to the literature. MATERIAL AND METHODS: In this study, MCF-7 breast cancer cell line was used and galantamine was obtained from Leucojum aestivum by HPLC method. The effect of galantamine on cell viability was determined by CCK-8 assay at 9 different doses insert ignore into journalissuearticles values(control, 10, 40, 70, 100, 130, 160, 190, 210 μg/ml); including the control group. Then, to understand the effect of galantamine, other assays insert ignore into journalissuearticles values(total antioxidant status insert ignore into journalissuearticles values(TAS);, total oxidant status insert ignore into journalissuearticles values(TOS);, poly-ADP ribose polymerase insert ignore into journalissuearticles values(PARP);, and vascular endothelial growth factor insert ignore into journalissuearticles values(VEGF); levels); were examined in 3 groups; control, LD50 insert ignore into journalissuearticles values(100 μg/ml); and high dose insert ignore into journalissuearticles values(210 μg/ml); group. The oxidative stress index insert ignore into journalissuearticles values(OSI); was then calculated. RESULTS: Galantamine decreased cell viability. The LD50 dose was determined as 100 μg/ml. There was no significant change in VEGF levels. There was a significant dose-dependent decrease in PARP levels insert ignore into journalissuearticles values(Control: 2.78667±0.155392, LD50: 1.51000±0.107145, High dose: 1.01000±0.054772 ng/L, p=0.000);. Compared to the control group, there was a significant dose-related decrease in TAS data insert ignore into journalissuearticles values(Control: 0.09633±0.002658, LD50: 0.06283±0.002317, High dose: 0.04050±0.001871, p=0.000); and a significant dose-related increase in TOS data insert ignore into journalissuearticles values(Control: 0.12500±0.010488, LD50: 0.21667±0.015055, High dose: 0.31833±0.021370, p=0.000);. A significant dose-related increase in OSI data was found. insert ignore into journalissuearticles values(Control: 129.964±13.018, LD50: 345.161±26.480, High dose: 788.485±78.575, p=0.000);. CONCLUSIONS: It was determined that galantamine had no significant effect on angiogenesis at any dose based on VEGF levels. Galantamine had a negative effect on cell viability and proliferation at certain doses to MCF-7 cells and was found to increase oxidative stress. The decrease in PARP levels indicates the possibility that cells may result in an apoptotic process. These findings may be useful to take a different approach to the use of galantamine in cancer research.
Keywords : Galantamin, Oksidatif stres, Poli ADP riboz, polimeraz, Vasküler endotelyal büyüme faktörü, MCF 7 hücreleri