The Effects of Restraint and Cold Restraint Stress on Coagulation Indicators in Wistar Albino Rats

Authors : Çağlasu KOÇ, Mehmet EKİCİ

Pages : 160-165

Doi:10.30607/kvj.1239878

View : 83 | Download : 108

Publication Date : 2023-06-30

Article Type : Research Paper

Abstract :The aim of this study was to investigate the effects of restraint and cold restraint stress from acute stress protocols on coagulation indicators in rats. The study was conducted in 18 male Wistar albino rats aged 8-10 weeks with a body weight of 180-220 g. After a one-week adaptation period, the rats were randomly divided into three groups insert ignore into journalissuearticles values(n=6/group);. The animals in the control group were not exposed to any stress. Rats in the restraint group were housed in restrainers designed for rats, and their movement was restricted for 2 hours at room temperature. Rats in the cold restraint group were kept in the restrainer at +4°C for 2 hours. Blood samples were collected by cardiac puncture under ketamine and xylazine anesthesia in Vacutainer® tubes containing 3.2% sodium citrate insert ignore into journalissuearticles values(0.109 M trisodium citrate);. Coagulation indicators insert ignore into journalissuearticles values(aPTT, PT, INR, fibrinogen, and D-dimer); were analyzed using an automated analyzer insert ignore into journalissuearticles values(Roche Cobas t511, Switzerland);. Although acute stress insert ignore into journalissuearticles values(restraint and cold restraint stress); had no effect on aPTT and D-dimer levels insert ignore into journalissuearticles values(p>0.05);, it increased PT and INR values insert ignore into journalissuearticles values(p<0.05); and decreased fibrinogen concentration insert ignore into journalissuearticles values(p<0.05);. Consequently, acute stress may lead to platelet hypofunction in rats by prolonging PT, increasing INR, and decreasing fibrinogen concentration.
Keywords : Akut stres, aPTT, D dimer, Fibrinojen, INR, PT