Investigation of the Effects of Rutin on Valproic Acid Induced Testicular Damage in Rats

Authors : Serkan Ali Akarsu, Nâzım Abdülkadir Kankiliç, Ender Erdoğan

Pages : 260-268

Doi:10.30607/kvj.1295945

View : 118 | Download : 211

Publication Date : 2023-09-30

Article Type : Research Paper

Abstract :Valproic acid (VALP) is a drug used for many psychiatric diseases such as epilepsy. However, the use of VALP has potential side effects on various tissues, including the testicles. Rutin (RUT) is a flavonoid with protective effects against oxidative stress-induced diseases and lipid peroxidation. In this study, the protective effects of RUT against testicular damage caused by VALP were investigated. For this purpose, 35 male Spraque-Dawley rats weighing 220-250 g were used in the study. The rats were randomly divided into 5 groups as Control (physiological saline), RUT (100 mg/kg/bw), VALP, (500mg/kg/bw), VALP+RUT 50 (500 mg/kg/bw VALP+50 mg/kg/bw RUT), and VALP +RUT 100 (500 mg/kg/bw VALP+100 mg/kg/bw RUT). At the end of the RUT and VALP administrations, the rats were sacrificed and testicular tissues were taken to be used for biochemical and spermatological analyzes. According to the results of this study, the MDA level in the testicular tissues of the VALP group was found to be statistically higher than the other experimental groups (p<0.05). Testicular tissue GSH and Nrf-2 level was the lowest in the VALP group. TNF-α, IL-1Β and MAPK14 levels in testicular tissue were highest in the VALP group, while it was decreased in the RUT treatment groups. Similarly, the highest levels of Bax, Caspase-3 and MMP-9 were observed in the VALP group, while RUT treatment decreased this value. When the spermatological analyzes were examined, it was observed that the total motility value decreased significantly in the VALP group, but the total motility value increased in the RUT treatment group (p<0.05). A significant increase in the rate of dead and abnormal sperm was found in the VALP group, but these parameters improved in the RUT treatment group. As a result, RUT has protective effects against VALP-induced testicular damage in rats.
Keywords : Oksidatif stres, rat, rutin, sperma, valproik asit