Evaluation of Postbiotics Belonging to Industrial Kefir-Derived Exopolysaccharide-Producing Lacticaseibacillus rhamnosus and Lacticaseibacillus paracasei as Anti-Candidal Biofilm Agents

Authors : Adalet Dışhan, Esmanur Abatay, Zafer Gonulalan

Pages : 215-226

View : 82 | Download : 77

Publication Date : 2025-10-03

Article Type : Research Paper

Abstract :Candidal biofilm is a concern in both medical and industrial fields. This study aims to reveal the effect of postbiotics on the transcriptional level of biofilm-associated genes of Candida albicans in exopolysaccharide-producing industrial kefir-derived isolates and to determine their biofilm prevention and treatment properties. In the study, the exopolysaccharide production capacity of lactic acid bacteria obtained from kefir samples of eight different brands from supermarkets was determined. The transcriptional effect of postbiotics of exopolysaccharide-producing isolates on C. albicans biofilm-related genes was carried out via reverse transcription-polymerase chain reaction. The biofilm prevention and biofilm treatment effects of postbiotics were determined in abiotic media (polystyrene microplate). The minimum inhibitory concentration (MIC) values of Lacticaseibacillus rhamnosus and Lacticaseibacillus paracasei (exopolysaccharide-producing isolates) postbiotics were determined as 12.5% and 25%, respectively. While 2xMIC and above doses of L. rhamnosus and L. paracasei postbiotics were effective in prevention, MIC and above doses were effective in biofilm treatment. als1, als3, and bcr genes were down-regulated at 2xMIC and MIC doses of L. rhamnosus postbiotic. At exposure to a 2xMIC dose of L. paracasei postbiotic, all genes examined were down-regulated. On average, 0.03, 0.15, and 0.79-fold downregulation of als1, als3 and bcr genes was determined at 24 hours at 2xMIC dose of L. rhamnosus postbiotic; 0.31, 0.55, and 0.77-fold downregulation was determined at 24 hours at MIC doses. A 0.39, 0.05, 0.91, and 0.04-fold downregulation of L. paracasei postbiotic at 2xMIC dose at 24 hours was determined in als1, als3, bcr and hwp genes, respectively. In conclusion, the discovery of new approaches that can prevent or treat biofilms could stimulate the emergence of novel bio-control agents.
Keywords : Biyofilm, Candida, Ekzopolisakkarit, Kefir, Lacticaseibacillus, Postbiyotikler

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