Age- and Sex- Dependent Changes in Serum Levels of TAS, TOS, TLR2, TLR4, HSP60, HSP90, and HMGB1

Authors : Salih Tunç KAYA

Pages : 105-115

Doi:10.18521/ktd.1214575

View : 34 | Download : 16

Publication Date : 2023-03-15

Article Type : Research Paper

Abstract :Objective: Cellular and physiological functions may be affected in an age- and sex-specific manner. The aim of this study is to investigate sex- and age-specific differences in the serum levels of Total Antioxidant Status insert ignore into journalissuearticles values(TAS);, Total Oxidant Status insert ignore into journalissuearticles values(TOS);, Oxidative Stress Index insert ignore into journalissuearticles values(OSI);, Toll-like Receptor 2 insert ignore into journalissuearticles values(TLR2);, Toll-Like Receptor 4 insert ignore into journalissuearticles values(TLR4);, Heat Shock Protein 60 insert ignore into journalissuearticles values(HSP60);, Heat Shock Protein 90 insert ignore into journalissuearticles values(HSP90);, and High Mobility Group Box 1 insert ignore into journalissuearticles values(HMGB1); as well as to examine the correlation between them. Methods: Four groups of mice, each including seven animals, were used in the present study: young males and females insert ignore into journalissuearticles values(6 months old);; old males and females insert ignore into journalissuearticles values(24 months old);. Blood samples were taken from the heart and serum was used to assay the levels of TLR2, TLR4, HSP60, HSP90, HMGB1, TAS and TOS. Results: HGMB1, TOS and OSI were higher in old females than in young females insert ignore into journalissuearticles values(p<0.05);. TLR2 and TLR4 levels were higher in young females than in young males; however, HSP60 was lower in young females than in young males insert ignore into journalissuearticles values(p<0.01);. HSP60 was lower in old males than in young males insert ignore into journalissuearticles values(p<0.05);. Positive correlations were present between TLR2, TLR4 and HMGB1 insert ignore into journalissuearticles values(p=0.001, r=0.096; p=0.012, r=0.867; p=0.002, r=0.935, respectively); as well as between HMGB1 and HSP60 insert ignore into journalissuearticles values(p=0.049, r=0.756); in young females. A negative correlation was detected between HSP90 and TLR4 in young males insert ignore into journalissuearticles values(p=0.000, r=-0,982);, and between HSP60 and TLR2, OSI in old males insert ignore into journalissuearticles values(p=0.014, r=-0.856; p=0.042, r=-0.772, respectively);. Conclusions: The results of present study indicated that age and sex could be important factors for the serum levels of TLR2, TLR4, HSP60, HSP90, HMGB1 and OSI as well as the correlation between them.
Keywords : Cinsiyet, Yaş, Yüksek Mobilite Grup Kutusu 1, Isı Şok Proteinleri, Toll Benzeri Reseptörler, Oksidatif Stres