Protective Effects of the Purinergic Receptor Antagonist A438079 on Lipopolysaccharide-Induced Kidney Injury in Rats

Authors : Nevra AYDEMİR CELEP, Seçkin ÖZKANLAR, Elif ERBAŞ, Adem KARA, Nergis ULAŞ

Pages : 6-11

View : 40 | Download : 12

Publication Date : 2023-03-29

Article Type : Research Paper

Abstract :Sepsis is an important clinical problem associated with organ dysfunction and high mortality. To investigate the effects of this purinergic receptor on renal dysfunction in sepsis, a rat model was created by the intraperitoneal injection of lipopolysaccharides. The groups were control, A438079, lipopolysaccharides, and lipopolysaccharide+A438079. Following lipopolysaccharide insert ignore into journalissuearticles values(8 mg/kg); injection, A438079 insert ignore into journalissuearticles values(15 mg/kg); was administrated insert ignore into journalissuearticles values(intraperitoneally); in the study groups. Then, blood and kidney tissues were collected for analysis from rats sacrificed under anesthesia. In the histopathological evaluations, it was observed that A438079 reduced the damage caused by sepsis. It was observed that the creatinine and blood urea nitrogen); concentrations of the serum samples belonging to the lipopolysaccharide+A438079 group were lower than the serum samples belonging to the lipopolysaccharide group. Aquaporin-2, Bcl-2, caspase-3, and NfkB-p65 protein expressions in kidney tissues were evaluated by western blot. On the other hand, it was observed that there was a decrease in the expression of these proteins in the A438079-treated groups. In conclusion, A438079 can reduce the inflammatory response in acute kidney injury induced by lipopolysaccharides and improve renal dysfunction.
Keywords : A438079, LPS, purinergic receptor, rats, sepsis

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