Variability of CAPN1 g.5709 C>G and MYF5 g.1911 A>G Polymorphisms in Beef Cattle Imported from Brazil to Turkey

Authors : Sena ARDIÇLI, Hakan USTÜNER, Öznur ARSLAN, Oğuz KANDAZOĞLU

Pages : 72-78

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Publication Date : 2019-12-13

Article Type : Research Paper

Abstract :The objective of the present study was to determine genotypic/allelic frequencies and population genetic indices of CAPN1 g.5709 C>G and MYF5 g.1911 A>G polymorphisms in beef cattle imported from Brazil to Turkey. Single nucleotide polymorphisms were carried out using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis. A total of 108 bulls, including Angus, Angus×Hereford×Nellore, Brahman, Hereford, Limousine, and Charolais breeds, were genotyped. Concerning the CAPN1, CC genotype was not found in this study. Besides, the G allele frequency was quite high (0.75). Regarding the MYF5, frequency of AA genotype was rather low (4.63%) compared to the other two genotypes, AG and GG. Therefore, the frequency of A allele was quite low (0.21). On the basis of breed-specific evaluation, the highest frequency of GG genotype of the CAPN1 was found in Brahman breed (88.24%) whereas the highest frequency of heterozygous genotype was determined in Charolais breed (85.71%). The frequency of MYF5 GG genotype was found to be very high in Limousine breed (75.00%). Moreover, MYF5 AA genotype was absent in Angus, Brahman, Limousine, and Charolais breeds. The present study may be useful for further genetic analyses conducted on beef cattle imported into Turkey.
Keywords : Beef cattle, CAPN1, MYF5