Assessing Antifungal Resistance and Biofilm-Forming Capacity of Candida parapsilosis Isolates from Makeup Sponges

Authors : Gülçin Özcan Ateş, Tuğba Hanedan

Pages : 81-89

Doi:10.30708/mantar.1656079

View : 22 | Download : 36

Publication Date : 2025-04-30

Article Type : Research Paper

Abstract :Candida parapsilosis is an opportunistic pathogen that can colonize human mucous membranes and skin and develop resistance to antifungal treatments due to its ability to form biofilms. This study investigated the biofilm formation capacities and antifungal resistance of Candida parapsilosis isolates obtained from makeup sponges. The biofilm formation capacities among 46 Candida parapsilosis isolates were determined using the crystal violet quantitative method. Amphotericin B resistance in the isolates was evaluated with the agar disk diffusion method. The antifungal resistances of biofilm-producing species were assessed using the gradient test method. The crystal violet analysis revealed that most isolates were weak biofilm producers, with 17 exhibiting moderate biofilm production. It was determined that the isolates gave inhibition zones between 19.73 ± 1.54 mm and 23.71 ± 0.70 mm against amphotericin B by the agar disk diffusion method. It was concluded that 17 isolates with moderate biofilm production were susceptible to the antifungal agents clotrimazole, fluconazole, itraconazole, amphotericin B, and nystatin. The study emphasizes that makeup sponges may serve as reservoirs for Candida parapsilosis and highlights the importance of evaluating the antifungal susceptibility of biofilm-forming isolates. These findings are significant for the hygienic use of personal care products and implementing infection control measures.
Keywords : Candida parapsilosis, Makyaj süngeri, Antifungal direnç, Biyofilm