Assessing the susceptibility of some gut bacteria to the extract from needles of Turkish pine

Authors : Ahu DEMİRTAŞ

Pages : 1-6

Doi:10.24998/maeusabed.869812

View : 23 | Download : 12

Publication Date : 2021-04-30

Article Type : Research Paper

Abstract :Plant extracts have the potential to be safe alternatives to antibiotics that disrupt the gut flora. The aim of the present study was to assess the susceptibility of some gut bacteria to the extract from needles of Turkish pine insert ignore into journalissuearticles values(Pinus brutia Ten.); using microdilution method in an anaerobic chamber. Turkish pine needle extract promoted the growth of Bifidobacterium bifidum, Bifidobacterium infantis, and Lactobacillus acidophilus from gut commensals at 0.2-6.25 mg/mL, 0.4-6.25 mg/mL, and 0.4-1.6 mg/mL dose ranges, respectively insert ignore into journalissuearticles values(P<0.05);. However, the extract had a potential inhibitory activity on Bifidobacterium species starting from 12.5 mg/mL, on L. acidophilus starting from 6.25 mg/mL, and L. casei starting from 3.13 mg/mL concentrations insert ignore into journalissuearticles values(P<0.05);. Minimal inhibitor concentration insert ignore into journalissuearticles values(MIC); was 25 mg/mL for all commensal species. Turkish pine needle extract also showed a potential inhibitory activity against gut pathogens Escherichia coli and Clostridium perfringens from 0.4 mg/mL and against Staphylococcus aureus and Fusobacterium nucleatum from 0.8 mg/mL dose insert ignore into journalissuearticles values(P<0.05);. The MICs were 6.25, 12.5, 25, and 50 mg/mL for S. aureus, F. nucleatum, E. coli, and C. perfringens, respectively. It was concluded that using the Turkish pine needle extract in a dose range of 0.2-12.5 mg/mL, where it protected most of the commensal bacteria and was toxic against some of the pathogens, might produce desirable impacts in the gut.
Keywords : Antibacterial, Gut bacteria, MIC, Plant extracts, Turkish pine needle