The ameliorative effect of Acetylsalicylic acid plus Ascorbic acid against renal injury in Corn Syrup-fed rats

Authors : Şükriye YEŞİLOT, Halil AŞÇI, Meltem ÖZGÖÇMEN, Mustafa SAYGIN, İlkay ARMAĞAN, Ekrem ÇİÇEK

Pages : 26-33

Doi:10.24880/maeuvfd.981913

View : 30 | Download : 15

Publication Date : 2022-04-30

Article Type : Research Paper

Abstract :Dietary consumption of commercially prepared insert ignore into journalissuearticles values(often through processing); corn syrup can activate reactive oxygen species and the inflammatory pathways observed in kidney damage in humans and experimental animals. This study was designed to assess the effects of antioxidant properties of acetylsalicylic insert ignore into journalissuearticles values(ASA); and ascorbic acid insert ignore into journalissuearticles values(AA); on corn syrup insert ignore into journalissuearticles values(CS); induced renal injury. Forty male Sprague–Dawley rats insert ignore into journalissuearticles values(14–16 weeks); were randomly classified into 5 groups, each containing 8 rats: control group insert ignore into journalissuearticles values(C);, CS group, CS+ASA group insert ignore into journalissuearticles values(ASA-10 mg/kg/day/po);, CS+AA group insert ignore into journalissuearticles values(AA-200 mg/kg/day/po); and CS+ASA+AA group insert ignore into journalissuearticles values(combination treatment with ASA and AA doses);. At the end of the experiment, blood and tissue samples were collected for biochemical, histopathological and immunohistochemical examinations. Malondialdehyde insert ignore into journalissuearticles values(p<0.001); levels were increased in the CS group compared with control group and were decreased in the ASA, AA and ASA+AA treated groups insert ignore into journalissuearticles values(p≤0.001 for all); compared with CS group. Catalase activities were decreased in the CS group insert ignore into journalissuearticles values(p≤0.001); compared with the control group insert ignore into journalissuearticles values(p<0.001); and significantly increased in AS+AA-treated group insert ignore into journalissuearticles values(p<0.001); compared with the CS group. Significant histopathological changes including tubular vacuoler degeneration, tubular dilatation, cortical and medullar haemorrhage, mononuclear cell infiltration, and increased inducible nitric oxide synthase and tumor necrosis factor-α staining were observed in the CS group. Combination theraphy reversed all these changes. The administration of ASA and AA for the treatment of kidney damage due to corn syrup consumption provides an ideal target for improving oxidative stress and potential therapeutic treatments.
Keywords : Acetylsalicylic acid, Ascorbic acid, Corn syrup, Renal injury