Postbiotic metabolites of probiotic Lactiplantibacillus plantarum in natural yoghurts from Muş region and their effects on colon cancer cell lines

Authors : Yusuf Alan, Ahmet Savcı

Pages : 238-246

Doi:10.18586/msufbd.1696058

View : 54 | Download : 97

Publication Date : 2025-12-24

Article Type : Research Paper

Abstract :Probiotic Lactic Acid Bacteria (LAB) can exhibit different characteristics depending on their source of isolation, and they may also possess varying potentials in terms of postbiotic metabolite amounts and anticancer activity. Therefore, 22 of 125 LAB isolates isolated from yogurt were determined to be like Lactiplantibacillus plantarum using MALDI-TOF MS. When the probiotic resistance of L. plantarum isolates to different pH, pepsin, pancreatin, and bile salts was investigated, all isolates were found to show good activity. The amount of postbiotic metabolites of six L. plantarum strains (3, 7, 13, 25, 26, and 29) and L. plantarum STD, which showed good probiotic properties, was determined by HPLC. Tartaric acid, pyruvic acid, and malic acid synthesis were not detected in these isolates. It was determined that these isolates synthesized the most acetic acid and the least succinic acid. Furthermore, it was observed that these isolates exhibited improved anticancer activity on colon cancer cell lines (HT-29 and Caco-2), depending on increasing concentrations. It is believed that L. plantarum isolates will contribute to the food and health industries with further support from new in vivo studies.
Keywords : Antikanser, LAB, Lactiplantibacillus plantarum, Postbiyotik metabolit, Probiyotik

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