Green Synthesis of Silver Nanoparticles Using Safran (Crocus sativus) Purple flower and Their Antimicrobial activity

Authors : Mehmet Fırat BARAN, Hatice BECEREKLİ, Ülkü KARAMAN

Pages : 8-17

Doi:10.56941/odutip.1285154

View : 70 | Download : 68

Publication Date : 2023-04-30

Article Type : Research Paper

Abstract :Objective: In this research, CS-AgNPs were created, described, and their antibacterial activity assessed utilizing an inexpensive, simple, and ecologically friendly extraction approach from the waste saffron flower\`s purple flower parts. Methods: : In this study, silver nanoparticles insert ignore into journalissuearticles values(AgNPs); were synthesized quickly, cheaply, and environmentally friendly utilizing purple flower extract from saffron insert ignore into journalissuearticles values(Crocus sativus insert ignore into journalissuearticles values(CS););. For the explanation of the structure of silver nanoparticles synthesized with saffron flower extract insert ignore into journalissuearticles values(CS-AgNPs);; UV-visible insert ignore into journalissuearticles values(UV-vis.); Spectrophotometer, Fourier Scanning Electron Microscope insert ignore into journalissuearticles values(FE-SEM);, Scanning Electron Microscope insert ignore into journalissuearticles values(SEM);, Electron Distributed X-rays insert ignore into journalissuearticles values(EDX);, Fourier Transform Infrared Spectroscopy insert ignore into journalissuearticles values(FT-IR);, X-Ray Characterized using Diffraction Diffractrometer insert ignore into journalissuearticles values(XRD);, Transmission Electron Microscope insert ignore into journalissuearticles values(TEM);, Zeta potential data. It was discovered that the UV-visible spectrum of the biologically produced silver nanoparticles generated at a maximal wavelength of 405.68 nm in the spectrum collected after 15 minutes. Furthermore, it was noted that the synthesized nanoparticles\` UV spectrum, taken a month later, revealed the same wavelength and that the resulting nanoparticles were stable. CS-AgNPs\` antimicrobial effects against gram-positive, gram-negative, and fungal pathogens were assessed employing the minimum inhibition concentration approach. Results: The generated CS-AgNPs were found to be active against both fungi and bacteria as a consequence. Conclusion: It turned out that even at very low concentrations, the antimicrobial activity of silver nanoparticles enhanced with decreasing size and had high antibacterial and anticandidal implementation.
Keywords : Crocus sativus, CS AgNP, MİK, Antibakteriyel etki ve Antifungal etki