The Effect of Thymoquinone on the Protein Levels of PLA2G7, UCP2, and NEDD4L Genes Associated with Lipid Droplets Formation in Prostate Cancer

Authors : Abdelhak Hadjmimoune, Ahmet Çarhan, Beyza Ecem Öz Bedir, Hümeyra Yılmaz, Mustafa Emre Ercın, Ender Şimşek

Pages : 97-102

Doi:10.26453/otjhs.1422576

View : 103 | Download : 93

Publication Date : 2024-03-11

Article Type : Research Paper

Abstract :Objective: Prostate cancer (PCa) patients suffer severe side effects of standard treatment beside the resistance to castration. PCa cells shows increased lipogenesis. Thymoquinone (TQ) inhibits cell proliferation, metastasis, and invasion. However, there was no study on the effect of TQ on the levels of NEDDL4, PLA2G7, and UCP2 lipid droplets (LD) related proteins. Hence, the study aims to investigate the impact of TQ on PLA2G7, UCP2, and NEDD4L proteins on DU145 and PC3 cell lines. Materials and Methods: Cells were cultured and treated with TQ with a IC50 of 60 µM and 80 µM for DU145 and PC3, respectively. PLA2G7, UCP2, and NEDD4L levels were measured using the ELISA. Results: TQ has significantly increased the level of NEDD4L (p<0.01 for DU145 and p<0.001 for PC3) and decreased the level of UCP2 proteins (p<0.05). Conclusions: Our preliminary findings suggest that TQ may impact NEDD4L and UCP2, indicating a potential role in repressing LD. Further investigations are needed to confirm the efficacy of TQ and explore its potential utility as a therapeutic agent for PCa treatment.
Keywords : NEDD4L, PLA2G7, prostat kanser metabolizması, timokinon, UCP2