Strong association between COVID-19 and Single Nucleotide Polymorphism in Nuclear Factor Kappa B genes

Authors : Nil Özbilüm, Burcu Bayyurt, Sevgi Baltacı, Mehmet Bakır, Serdal Arslan

Pages : 15-21

Doi:10.26453/otjhs.1505688

View : 55 | Download : 68

Publication Date : 2025-03-17

Article Type : Research Paper

Abstract :Objectives: SARS-CoV-2 is an enveloped, positive-sense, single-stranded RNA virus belonging to the Betacoronavirus genus. This study aimed to investigate the relationship between COVID-19 severity and NF-κB1 -94 ins/del (rs28362491), NF-κB1A 3’UTR A/G (rs696), -826 C/T (rs2233406) polymorphisms. Materials and Methods: We investigated the frequencies of these gene polymorphisms in 150 patients with COVID-19 and 171 healthy controls. Total DNA was isolated from the blood samples, and then the PCR-RFLP study was used for genotyping. All statistical analyses were calculated using the chi-square method using SPSS. Results: A statistically significant differences were determined in the D allele frequency, WD and DD genotype frequencies for the rs28362491 polymorphism. For rs696 polymorphism, there was a statistically significant difference in the frequency of the G allele of patients with COVID-19. Additionally, for this polymorphism, CT and TT genotype frequencies were shown to be statistically significant. It was also found that the T allele, CT, and TT genotype frequencies for the rs2233406 have a statistically significant difference. Conclusion: A significant association was found between COVID-19 disease and NF-κB genes, but further studies, such as investigating promoter activity or gene expression levels, are needed.
Keywords : COVID-19, NF-κB1-94 Ins/Del ATTG, NF-κB1A 3, NF-κB1A -826 C/T, polimorifzm