Resveratrol Reorganizes the Impaired Cellular Functions of ARPE-19 Cells Created in Diabetes Model

Authors : Mehmet Argun, Ömer Çelik

Pages : 38-44

Doi:10.26453/otjhs.1576093

View : 41 | Download : 65

Publication Date : 2025-03-17

Article Type : Research Paper

Abstract :Objective: It is well known that high blood glucose levels can damage many visual functions. So, the study aimed to investigate the effects of resveratrol on cellular lipid peroxidation (MDA), cytokines, VEGF-A and apoptosis levels in vitro diabetes model-induced ARPE-19 cells. Materials and Methods: Six experimental groups were conceptualized as follows. 1-Control group: Received no treatment (Standard Growth Medium), 2-Mannitol Group (M): Cells incubated in 19.5 mM Mannitol supplemented medium, 3-High Glucose Group (HG): Cells incubated in high glucose (25 mM Glucose), 4-Resveratrol Group (R): Cells incubated with 100 µM resveratrol Standard Growth Medium, 5-Mannitol + Resveratrol Group (M+R), 6-High Glucose + Resveratrol Group (HG+R). In All groups, cells were incubated for 48 hrs, and MDA, IL-1β, TNF-α, VEGF-A and Apoptosis levels were measured. Results: High glucose medium increased the MDA, IL-1β, TNF-α and VEGF-A levels while resveratrol caused a significant decrement in MDA, IL-1β, TNF-α and VEGF-A levels in diabetes model-induced ARPE-19 cells. As a result, resveratrol prevented the ARPE-19 cells against diabetes related impaired conditions. Conclusions: In conclusion, resveratrol can reverse disrupted cellular functions by reducing cellular oxidative stress and supporting cellular viability.
Keywords : Apoptoz, ARPE-19, diyabet, resveratrol, VEGF-A