Bacillus sp. ZBP10 SUŞU ile DERİN KÜLTÜR FERMANTASYONUNDA AMİLAZ ÜRETİMİ
Authors : Ayşe AVCI, Dzhanser ADEM, İsmail İNAN, Samet YÜKSEL, Kübra Gizem ŞAHİN, Zekeriya KALKAN
Pages : 131-136
View : 35 | Download : 10
Publication Date : 2016-06-01
Article Type : Research Paper
Abstract :Bacillussp. ZBP10 is an amylase producing strain isolated from a soil sample collected from a potatocultivation field in Sakarya. In this study, culture conditions were optimized for Bacillus sp. ZBP10 inorder to increase amylase production using submerged fermentation. The effects of temperatureinsert ignore into journalissuearticles values(30-40°C);, fermentation time insert ignore into journalissuearticles values(24-72 h);, initial medium pH insert ignore into journalissuearticles values(6.0-9.0);, carbon sources insert ignore into journalissuearticles values(soluble, wheat,rice and corn starches); and substrate concentration insert ignore into journalissuearticles values(5-30 g/L); on the production of amylase weredetermined. According to the results, the bacterium produced maximum amount of amylase, when theinitial pH of the medium was 7.0, at 33°C, and within 48 h. Soluble starch was the best substrate amongthe starches tested. Optimum substrate concentration was 20 g/L for enzyme production with which3.57±0.19 U/mL enzymatic activity was obtainedKeywords : Amilaz, Bacillus, enzim, niflasta
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